Shedding light on photosynthesis at sea
Japanese research explains how submarine plants undergo photosynthesis given only blue-green light can reach them at such depths
Plants that live on land, such as spinach, grow by using sunlight to perform photosynthesis. How, then, do algae photosynthesise in the deep sea, an environment where only a little light reaches them?
Land plants mainly absorb red and blue light from the sun and use it for photosynthesis. However, only weak blue-green light reaches the ocean floor. Therefore, macroalgae growing in the ocean have developed a protein, a so-called photosynthetic antenna, that efficiently utilises this blue-green light.
The photosynthetic antenna of marine macroalgae is very similar to that of land plants but differs in the structure of the pigments bound to it.
Land plants have two types of pigments bound to their photosynthetic antennae, namely carotenoids and chlorophylls. In the marine green macroalga Codium fragile, the major carotenoids are substituted with siphonaxanthin while some chlorophyll a molecules are replaced by chlorophyll b molecules.
Siphonaxanthin and chlorophyll b are known to contribute to increased absorption of green light and blue-green light, respectively, but the mechanism has not yet been fully understood.
Responding to this gap, a research team led by Associate Professor Ritsuko Fujii, from the Research Center for Artificial Photosynthesis (ReCAP) at Osaka Metropolitan University, and graduate student Soichiro Seki, from the Graduate School of Science at Osaka City University, used cryogenic electron microscopy to investigate the structures and binding environments of pigments bound to the photosynthetic antenna of C. fragile.
The results allow for the elucidation of the molecular mechanism by which blue-green light – the only light available in deep seawater – is efficiently utilised for photosynthesis.
High-resolution analysis by cryogenic electron microscopy showed that siphonaxanthin in C. fragile is greatly distorted and forms hydrogen bonds with the surrounding protein at two locations. This structural feature is deemed a key factor in siphonaxanthin’s ability to absorb green light.
Additionally, the researchers successfully detected the difference between chlorophyll a and chlorophyll b, and they clarified several chlorophyll molecule substitution sites.
When the substitution occurs, the adjacent region of chlorophyll b clusters becomes wider, enabling better absorption of blue-green light.
In other words, the team was able to obtain information on the pigment co-ordinates, contributing to a better understanding of the mechanism of more efficient photosynthesis.
Fujii said: “I think increasing the utilisation of photosynthesis simply by changing the pigment structure would be a cost-effective strategy.
“Learning such survival strategies of organisms would lead to improved use of sunlight and the development of renewable energy sources for human beings.”
The findings are published in BBA Advances.
Image: Using cryogenic electron microscopy, Osaka Metropolitan University scientists found that siphonaxanthin of C. fragile is greatly distorted and forms hydrogen bonds with the surrounding protein at two locations. The researchers also clarified several chlorophyll molecule substitution sites. When the substitution occurs, the adjacent region of chlorophyll b clusters becomes wider, enabling more efficient absorption of blue-green light. These structural features allow C. fragile to better utilise blue-green light for photosynthesis. © Ritsuko Fujii/ Osaka Metropolitan University.